ez-chip kit Search Results


ez-chip kit  (Merck KGaA)
90
Merck KGaA ez-chip kit
Ez Chip Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ezchip kit  (Merck KGaA)
90
Merck KGaA ezchip kit
Ezchip Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ezchip kit - by Bioz Stars, 2026-09
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enzymatic chromatin immunoprecipitation kit ez chip tm no. 17-371  (Merck KGaA)
90
Merck KGaA enzymatic chromatin immunoprecipitation kit ez chip tm no. 17-371
STAT3 promoted osteoclast differentiation through regulation of NFATc1. A, NFATc1 expression in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). Three independent cell isolations were collected and were tested three times. Experiments were repeated more than three times independently. Error bars represent mean ± S.D.; *, p < 0.05. B, level of NFATc1 protein in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days) and analyzed by Western blotting. Proteins were tested in the same samples used in Fig. 4C. C, NFATc1 expression of BMMs infected with Ad-GFP or Ad-CRE and then cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). Three independent cell isolations were collected and were tested three times. Error bars represent mean ± S.D.; *, p < 0.05. D, level of NFATc1 protein in BMMs infected with Ad-GFP or Ad-CRE followed by culture with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days) and then analyzed by Western blotting. Proteins were tested in the same samples used in Fig. 4J. E, immunofluorescence staining of NFATc1 (green) and CTSK (red) in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL on day 5. Three pairs of Stat3Ctsk mice and their Stat3fl/fl littermates were included, and representative examples are shown. F, ratio of NFATc1+ cells in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL on day 5. Three pairs of Stat3Ctsk mice and their Stat3fl/fl littermates were included. Error bars represent mean ± S.D.; *, p < 0.05. G, effect of STAT3 on activation of the NFATc1 promoter and evaluated using a luciferase expression reporter system performed in HEK293T cells. Four independent cell isolations were collected and tested. Error bars represent mean ± S.D.; *, p < 0.05. H, chromatin <t>immunoprecipitation</t> (ChIP)-qPCR analysis of STAT3 in NFATc1 promoter in RAW264.7 cells. Immunoprecipitation was performed with anti-STAT3, and IgG was used as a negative control. Precipitated DNA was detected by qPCR with specific primers. I, 293T cells were transfected with NFATc1-Luc, together with or without STAT3 or c-Fos. Three independent cell isolations were collected and tested. Error bars represent mean ± S.D.; *, p < 0.05. J, co-immunoprecipitation analysis of STAT3 with c-Fos was performed. A FLAG–c-Fos expression plasmid was co-transfected with Myc-STAT3 into 293T cells. Whole cell lysates (WCL) were used for immunoprecipitation (IP) and then immunoblotting (IB) with the indicated antibodies. Immunoprecipitation products were detected by Western blotting with the indicated antibodies.
Enzymatic Chromatin Immunoprecipitation Kit Ez Chip Tm No. 17 371, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ez-chip+kit/enzymatic+chromatin+immunoprecipitation+kit+ez+chip+tm+no++17+371/pmc06802509-519-12-21
Average 90 stars, based on 1 article reviews
enzymatic chromatin immunoprecipitation kit ez chip tm no. 17-371 - by Bioz Stars, 2026-09
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ez chip tm kit  (Merck KGaA)
90
Merck KGaA ez chip tm kit
STAT3 promoted osteoclast differentiation through regulation of NFATc1. A, NFATc1 expression in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). Three independent cell isolations were collected and were tested three times. Experiments were repeated more than three times independently. Error bars represent mean ± S.D.; *, p < 0.05. B, level of NFATc1 protein in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days) and analyzed by Western blotting. Proteins were tested in the same samples used in Fig. 4C. C, NFATc1 expression of BMMs infected with Ad-GFP or Ad-CRE and then cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). Three independent cell isolations were collected and were tested three times. Error bars represent mean ± S.D.; *, p < 0.05. D, level of NFATc1 protein in BMMs infected with Ad-GFP or Ad-CRE followed by culture with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days) and then analyzed by Western blotting. Proteins were tested in the same samples used in Fig. 4J. E, immunofluorescence staining of NFATc1 (green) and CTSK (red) in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL on day 5. Three pairs of Stat3Ctsk mice and their Stat3fl/fl littermates were included, and representative examples are shown. F, ratio of NFATc1+ cells in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL on day 5. Three pairs of Stat3Ctsk mice and their Stat3fl/fl littermates were included. Error bars represent mean ± S.D.; *, p < 0.05. G, effect of STAT3 on activation of the NFATc1 promoter and evaluated using a luciferase expression reporter system performed in HEK293T cells. Four independent cell isolations were collected and tested. Error bars represent mean ± S.D.; *, p < 0.05. H, chromatin <t>immunoprecipitation</t> (ChIP)-qPCR analysis of STAT3 in NFATc1 promoter in RAW264.7 cells. Immunoprecipitation was performed with anti-STAT3, and IgG was used as a negative control. Precipitated DNA was detected by qPCR with specific primers. I, 293T cells were transfected with NFATc1-Luc, together with or without STAT3 or c-Fos. Three independent cell isolations were collected and tested. Error bars represent mean ± S.D.; *, p < 0.05. J, co-immunoprecipitation analysis of STAT3 with c-Fos was performed. A FLAG–c-Fos expression plasmid was co-transfected with Myc-STAT3 into 293T cells. Whole cell lysates (WCL) were used for immunoprecipitation (IP) and then immunoblotting (IB) with the indicated antibodies. Immunoprecipitation products were detected by Western blotting with the indicated antibodies.
Ez Chip Tm Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ez chip tm kit - by Bioz Stars, 2026-09
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ez chip kit merk 17–371  (Merck KGaA)
90
Merck KGaA ez chip kit merk 17–371
STAT3 promoted osteoclast differentiation through regulation of NFATc1. A, NFATc1 expression in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). Three independent cell isolations were collected and were tested three times. Experiments were repeated more than three times independently. Error bars represent mean ± S.D.; *, p < 0.05. B, level of NFATc1 protein in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days) and analyzed by Western blotting. Proteins were tested in the same samples used in Fig. 4C. C, NFATc1 expression of BMMs infected with Ad-GFP or Ad-CRE and then cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). Three independent cell isolations were collected and were tested three times. Error bars represent mean ± S.D.; *, p < 0.05. D, level of NFATc1 protein in BMMs infected with Ad-GFP or Ad-CRE followed by culture with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days) and then analyzed by Western blotting. Proteins were tested in the same samples used in Fig. 4J. E, immunofluorescence staining of NFATc1 (green) and CTSK (red) in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL on day 5. Three pairs of Stat3Ctsk mice and their Stat3fl/fl littermates were included, and representative examples are shown. F, ratio of NFATc1+ cells in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL on day 5. Three pairs of Stat3Ctsk mice and their Stat3fl/fl littermates were included. Error bars represent mean ± S.D.; *, p < 0.05. G, effect of STAT3 on activation of the NFATc1 promoter and evaluated using a luciferase expression reporter system performed in HEK293T cells. Four independent cell isolations were collected and tested. Error bars represent mean ± S.D.; *, p < 0.05. H, chromatin <t>immunoprecipitation</t> (ChIP)-qPCR analysis of STAT3 in NFATc1 promoter in RAW264.7 cells. Immunoprecipitation was performed with anti-STAT3, and IgG was used as a negative control. Precipitated DNA was detected by qPCR with specific primers. I, 293T cells were transfected with NFATc1-Luc, together with or without STAT3 or c-Fos. Three independent cell isolations were collected and tested. Error bars represent mean ± S.D.; *, p < 0.05. J, co-immunoprecipitation analysis of STAT3 with c-Fos was performed. A FLAG–c-Fos expression plasmid was co-transfected with Myc-STAT3 into 293T cells. Whole cell lysates (WCL) were used for immunoprecipitation (IP) and then immunoblotting (IB) with the indicated antibodies. Immunoprecipitation products were detected by Western blotting with the indicated antibodies.
Ez Chip Kit Merk 17–371, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ez-chip+kit/ez+chip+kit+merk+17+371/pmc08807281-68-8-11
Average 90 stars, based on 1 article reviews
ez chip kit merk 17–371 - by Bioz Stars, 2026-09
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ezchip kit  (Merck & Co)
86
Merck & Co ezchip kit
STAT3 promoted osteoclast differentiation through regulation of NFATc1. A, NFATc1 expression in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). Three independent cell isolations were collected and were tested three times. Experiments were repeated more than three times independently. Error bars represent mean ± S.D.; *, p < 0.05. B, level of NFATc1 protein in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days) and analyzed by Western blotting. Proteins were tested in the same samples used in Fig. 4C. C, NFATc1 expression of BMMs infected with Ad-GFP or Ad-CRE and then cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). Three independent cell isolations were collected and were tested three times. Error bars represent mean ± S.D.; *, p < 0.05. D, level of NFATc1 protein in BMMs infected with Ad-GFP or Ad-CRE followed by culture with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days) and then analyzed by Western blotting. Proteins were tested in the same samples used in Fig. 4J. E, immunofluorescence staining of NFATc1 (green) and CTSK (red) in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL on day 5. Three pairs of Stat3Ctsk mice and their Stat3fl/fl littermates were included, and representative examples are shown. F, ratio of NFATc1+ cells in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL on day 5. Three pairs of Stat3Ctsk mice and their Stat3fl/fl littermates were included. Error bars represent mean ± S.D.; *, p < 0.05. G, effect of STAT3 on activation of the NFATc1 promoter and evaluated using a luciferase expression reporter system performed in HEK293T cells. Four independent cell isolations were collected and tested. Error bars represent mean ± S.D.; *, p < 0.05. H, chromatin <t>immunoprecipitation</t> (ChIP)-qPCR analysis of STAT3 in NFATc1 promoter in RAW264.7 cells. Immunoprecipitation was performed with anti-STAT3, and IgG was used as a negative control. Precipitated DNA was detected by qPCR with specific primers. I, 293T cells were transfected with NFATc1-Luc, together with or without STAT3 or c-Fos. Three independent cell isolations were collected and tested. Error bars represent mean ± S.D.; *, p < 0.05. J, co-immunoprecipitation analysis of STAT3 with c-Fos was performed. A FLAG–c-Fos expression plasmid was co-transfected with Myc-STAT3 into 293T cells. Whole cell lysates (WCL) were used for immunoprecipitation (IP) and then immunoblotting (IB) with the indicated antibodies. Immunoprecipitation products were detected by Western blotting with the indicated antibodies.
Ezchip Kit, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ez-chip chromati n immunopr ecipitation kit  (Merck KGaA)
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Merck KGaA ez-chip chromati n immunopr ecipitation kit
STAT3 promoted osteoclast differentiation through regulation of NFATc1. A, NFATc1 expression in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). Three independent cell isolations were collected and were tested three times. Experiments were repeated more than three times independently. Error bars represent mean ± S.D.; *, p < 0.05. B, level of NFATc1 protein in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days) and analyzed by Western blotting. Proteins were tested in the same samples used in Fig. 4C. C, NFATc1 expression of BMMs infected with Ad-GFP or Ad-CRE and then cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). Three independent cell isolations were collected and were tested three times. Error bars represent mean ± S.D.; *, p < 0.05. D, level of NFATc1 protein in BMMs infected with Ad-GFP or Ad-CRE followed by culture with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days) and then analyzed by Western blotting. Proteins were tested in the same samples used in Fig. 4J. E, immunofluorescence staining of NFATc1 (green) and CTSK (red) in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL on day 5. Three pairs of Stat3Ctsk mice and their Stat3fl/fl littermates were included, and representative examples are shown. F, ratio of NFATc1+ cells in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL on day 5. Three pairs of Stat3Ctsk mice and their Stat3fl/fl littermates were included. Error bars represent mean ± S.D.; *, p < 0.05. G, effect of STAT3 on activation of the NFATc1 promoter and evaluated using a luciferase expression reporter system performed in HEK293T cells. Four independent cell isolations were collected and tested. Error bars represent mean ± S.D.; *, p < 0.05. H, chromatin <t>immunoprecipitation</t> (ChIP)-qPCR analysis of STAT3 in NFATc1 promoter in RAW264.7 cells. Immunoprecipitation was performed with anti-STAT3, and IgG was used as a negative control. Precipitated DNA was detected by qPCR with specific primers. I, 293T cells were transfected with NFATc1-Luc, together with or without STAT3 or c-Fos. Three independent cell isolations were collected and tested. Error bars represent mean ± S.D.; *, p < 0.05. J, co-immunoprecipitation analysis of STAT3 with c-Fos was performed. A FLAG–c-Fos expression plasmid was co-transfected with Myc-STAT3 into 293T cells. Whole cell lysates (WCL) were used for immunoprecipitation (IP) and then immunoblotting (IB) with the indicated antibodies. Immunoprecipitation products were detected by Western blotting with the indicated antibodies.
Ez Chip Chromati N Immunopr Ecipitation Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


STAT3 promoted osteoclast differentiation through regulation of NFATc1. A, NFATc1 expression in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). Three independent cell isolations were collected and were tested three times. Experiments were repeated more than three times independently. Error bars represent mean ± S.D.; *, p < 0.05. B, level of NFATc1 protein in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days) and analyzed by Western blotting. Proteins were tested in the same samples used in Fig. 4C. C, NFATc1 expression of BMMs infected with Ad-GFP or Ad-CRE and then cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). Three independent cell isolations were collected and were tested three times. Error bars represent mean ± S.D.; *, p < 0.05. D, level of NFATc1 protein in BMMs infected with Ad-GFP or Ad-CRE followed by culture with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days) and then analyzed by Western blotting. Proteins were tested in the same samples used in Fig. 4J. E, immunofluorescence staining of NFATc1 (green) and CTSK (red) in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL on day 5. Three pairs of Stat3Ctsk mice and their Stat3fl/fl littermates were included, and representative examples are shown. F, ratio of NFATc1+ cells in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL on day 5. Three pairs of Stat3Ctsk mice and their Stat3fl/fl littermates were included. Error bars represent mean ± S.D.; *, p < 0.05. G, effect of STAT3 on activation of the NFATc1 promoter and evaluated using a luciferase expression reporter system performed in HEK293T cells. Four independent cell isolations were collected and tested. Error bars represent mean ± S.D.; *, p < 0.05. H, chromatin immunoprecipitation (ChIP)-qPCR analysis of STAT3 in NFATc1 promoter in RAW264.7 cells. Immunoprecipitation was performed with anti-STAT3, and IgG was used as a negative control. Precipitated DNA was detected by qPCR with specific primers. I, 293T cells were transfected with NFATc1-Luc, together with or without STAT3 or c-Fos. Three independent cell isolations were collected and tested. Error bars represent mean ± S.D.; *, p < 0.05. J, co-immunoprecipitation analysis of STAT3 with c-Fos was performed. A FLAG–c-Fos expression plasmid was co-transfected with Myc-STAT3 into 293T cells. Whole cell lysates (WCL) were used for immunoprecipitation (IP) and then immunoblotting (IB) with the indicated antibodies. Immunoprecipitation products were detected by Western blotting with the indicated antibodies.

Journal: The Journal of Biological Chemistry

Article Title: STAT3 controls osteoclast differentiation and bone homeostasis by regulating NFATc1 transcription

doi: 10.1074/jbc.RA119.010139

Figure Lengend Snippet: STAT3 promoted osteoclast differentiation through regulation of NFATc1. A, NFATc1 expression in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). Three independent cell isolations were collected and were tested three times. Experiments were repeated more than three times independently. Error bars represent mean ± S.D.; *, p < 0.05. B, level of NFATc1 protein in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days) and analyzed by Western blotting. Proteins were tested in the same samples used in Fig. 4C. C, NFATc1 expression of BMMs infected with Ad-GFP or Ad-CRE and then cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). Three independent cell isolations were collected and were tested three times. Error bars represent mean ± S.D.; *, p < 0.05. D, level of NFATc1 protein in BMMs infected with Ad-GFP or Ad-CRE followed by culture with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days) and then analyzed by Western blotting. Proteins were tested in the same samples used in Fig. 4J. E, immunofluorescence staining of NFATc1 (green) and CTSK (red) in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL on day 5. Three pairs of Stat3Ctsk mice and their Stat3fl/fl littermates were included, and representative examples are shown. F, ratio of NFATc1+ cells in WT and Stat3Ctsk BMMs cultured with M-CSF and RANKL on day 5. Three pairs of Stat3Ctsk mice and their Stat3fl/fl littermates were included. Error bars represent mean ± S.D.; *, p < 0.05. G, effect of STAT3 on activation of the NFATc1 promoter and evaluated using a luciferase expression reporter system performed in HEK293T cells. Four independent cell isolations were collected and tested. Error bars represent mean ± S.D.; *, p < 0.05. H, chromatin immunoprecipitation (ChIP)-qPCR analysis of STAT3 in NFATc1 promoter in RAW264.7 cells. Immunoprecipitation was performed with anti-STAT3, and IgG was used as a negative control. Precipitated DNA was detected by qPCR with specific primers. I, 293T cells were transfected with NFATc1-Luc, together with or without STAT3 or c-Fos. Three independent cell isolations were collected and tested. Error bars represent mean ± S.D.; *, p < 0.05. J, co-immunoprecipitation analysis of STAT3 with c-Fos was performed. A FLAG–c-Fos expression plasmid was co-transfected with Myc-STAT3 into 293T cells. Whole cell lysates (WCL) were used for immunoprecipitation (IP) and then immunoblotting (IB) with the indicated antibodies. Immunoprecipitation products were detected by Western blotting with the indicated antibodies.

Article Snippet: ChIP and qPCR ChIP analysis in RAW264.7 cells was performed using an enzymatic chromatin immunoprecipitation kit (EZ ChIP TM no. 17-371, Merck-Millipore, Darmstadt, Germany) following the manufacturer's instructions.

Techniques: Expressing, Cell Culture, Western Blot, Infection, Immunofluorescence, Staining, Activation Assay, Luciferase, Chromatin Immunoprecipitation, ChIP-qPCR, Immunoprecipitation, Negative Control, Transfection, Plasmid Preparation